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1.
Photodiagnosis Photodyn Ther ; 42: 103575, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37080349

RESUMO

Visceral leishmaniasis (VL) is a zoonotic disease caused by the protozoan Leishmania infantum, and dogs are considered the main urban hosts for future disease transmission. The first and most effective control against the spread of disease relies on identifying infected animals, followed by their treatment or sacrifice, to reduce the protozoan reservoirs. Despite the availability of various diagnostic tests for VL in dogs the development of a quick and accurate diagnosis is essential from a public health and ethical point of view. Here we analyze the use of UV-Vis spectroscopy as an alternative diagnostic method for VL diagnosis by using the antigen-antibody interaction in canine blood serum and machine learning algorithms. The main UV spectra in the 220 to 280 nm range exhibit nine electronic absorption bands, but no significative difference could be identified between the positive and negative group spectra. Finally, UV pre-proceed spectra by SNV (standard normal variate) were submitted to principal component analysis followed by Linear SVM algorithm, the prediction model was tested in a leave-one-out cross-validation and external validation test reaching an overall accuracy of 75%.


Assuntos
Doenças do Cão , Leishmaniose Visceral , Fotoquimioterapia , Animais , Cães , Leishmaniose Visceral/diagnóstico , Leishmaniose Visceral/veterinária , Soro , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes , Análise Espectral , Doenças do Cão/diagnóstico
2.
Data Brief ; 47: 109009, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36923019

RESUMO

This dataset describes the analysis of aflatoxins, macroelement and microelement concentration, oxidative stability and fatty acid profile of infant formula milk powder. Gas chromatography (CG) was used to identity 14 fatty acid methyl esters in in five samples of oils. The Racimat 893 method (induction times), Thermogravimetry (TG), Derivative Thermogravimetry (DTG) and Differential Scanning Calorimetry (DSC) were used to estimate the oxidative stability of oils. In addition, UV-VIS spectroscopic techniques were employed to obtain graphs of the absorption of each oil. The data presented can be useful in identifying compounds available in oils used to promote wound healing and understand the degradation mechanism.

3.
Rev Bras Parasitol Vet ; 29(1): e018019, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049147

RESUMO

The aim of the present study was to detect Cercopithifilaria bainae and other tick-borne pathogens and to perform molecular characterization of the tick Rhipicephalus sanguineus s.l. collected from dogs. Ticks (n = 432, including 8 larvae, 59 nymphs, and 365 adults) were sampled from domiciled dogs (n = 73) living in Campo Grande, Mato Grosso do Sul (Midwest Brazil). All ticks were morphologically identified as R. sanguineus. Genomic DNA was extracted in pools (three to five ticks per animal) and was used for definition of R. sanguineus haplotypes (based on 16S rRNA analysis) and pathogen identification (Cercopithifilaria sp., Ehrlichia canis, Anaplasma platys, Hepatozoon canis, Babesia vogeli and Rickettsia spp.). Rhipicephal us sanguineus specimens were identified as haplotypes A and B. DNA of Cercopithifilaria bainae (43.83%; 32/73), Ehrlichia canis (24.65%; 18/73), Anaplasma platys (19.17%; 14/73), and Hepatozoon canis (5.47%; 4/73) was detected. The identity of pathogens was confirmed by DNA sequence analysis. The present study confirms the presence of haplotypes A and B of R. sanguineus in the state of Mato Grosso do Sul and its importance as a vector of several pathogens of veterinary concern. Finally, this is the first report to identify C. bainae in ticks in the Midwestern region of Brazil.


Assuntos
Vetores Aracnídeos/parasitologia , Cães/parasitologia , Rhipicephalus sanguineus/parasitologia , Anaplasma/genética , Anaplasma/isolamento & purificação , Animais , Babesia/genética , Babesia/isolamento & purificação , Brasil , Ehrlichia canis/genética , Ehrlichia canis/isolamento & purificação , Eucoccidiida/genética , Eucoccidiida/isolamento & purificação , RNA Ribossômico 16S/genética , Rickettsia/genética , Rickettsia/isolamento & purificação
4.
J Wildl Dis ; 56(2): 288-293, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31880987

RESUMO

The growing interest in wild birds as pets and the increasingly complex interspecific relationships due to human activities in wild environments underscore the need for better knowledge about the health of these animals. Salmonella stands out among the infectious agents of considerable importance to both animal and human health. The importance of these enterobacteria to the health of humans and livestock animals has long been known. In wild birds in countries such as Brazil, however, little is known regarding the frequency of infection and the main serotypes of occurrence. In the present study, the frequency of infection and the main serotypes of Salmonella spp. were investigated in 258 birds at a wild animal rehabilitation center using conventional microbiological methods and molecular diagnostic techniques. Four birds infected with Salmonella enterica were identified using PCR. The birds were of the species Brotogeris chiriri, Ara ararauna, and Eupsittula aurea. Sequencing of DNA revealed identity with the Javiana, Newport, and Arizonae serotypes. These results are of considerable importance to the implementation of management and control measures directed at human and animal health.


Assuntos
Doenças das Aves/microbiologia , Papagaios , Salmonelose Animal/microbiologia , Salmonella/isolamento & purificação , Animais , Animais Selvagens , Doenças das Aves/epidemiologia , Brasil/epidemiologia , Salmonelose Animal/epidemiologia
5.
Rev. bras. parasitol. vet ; 29(1): e018019, 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1058020

RESUMO

Abstract The aim of the present study was to detect Cercopithifilaria bainae and other tick-borne pathogens and to perform molecular characterization of the tick Rhipicephalus sanguineus s.l. collected from dogs. Ticks (n = 432, including 8 larvae, 59 nymphs, and 365 adults) were sampled from domiciled dogs (n = 73) living in Campo Grande, Mato Grosso do Sul (Midwest Brazil). All ticks were morphologically identified as R. sanguineus. Genomic DNA was extracted in pools (three to five ticks per animal) and was used for definition of R. sanguineus haplotypes (based on 16S rRNA analysis) and pathogen identification (Cercopithifilaria sp., Ehrlichia canis, Anaplasma platys, Hepatozoon canis, Babesia vogeli and Rickettsia spp.). Rhipicephal us sanguineus specimens were identified as haplotypes A and B. DNA of Cercopithifilaria bainae (43.83%; 32/73), Ehrlichia canis (24.65%; 18/73), Anaplasma platys (19.17%; 14/73), and Hepatozoon canis (5.47%; 4/73) was detected. The identity of pathogens was confirmed by DNA sequence analysis. The present study confirms the presence of haplotypes A and B of R. sanguineus in the state of Mato Grosso do Sul and its importance as a vector of several pathogens of veterinary concern. Finally, this is the first report to identify C. bainae in ticks in the Midwestern region of Brazil.


Resumo O objetivo do presente estudo foi detectar Cercopithifilaria bainae e outros patógenos transmitidos por carrapatos e realizar a caracterização molecular do carrapato Rhipicephalus sanguineus s.l. coletado em cães. Carrapatos (n = 432, incluindo 8 larvas, 59 ninfas e 365 adultos) foram amostrados de cães domiciliados (n = 73) residentes no município de Campo Grande, Mato Grosso do Sul (centro-oeste do Brasil). Todos os carrapatos foram identificados morfologicamente como R. sanguineus. O DNA genômico foi extraído em pools (três a cinco carrapatos por animal), seguido pela definição de haplótipos (com base no gene 16S rRNA) e pela investigação de patógenos (Cercopithifilaria sp., Ehrlichia canis, Anaplasma platys, Hepatozoon canis, Babesia vogeli e Rickettsia spp.). Os espécimes coletados foram identificados como haplótipos A e B de R. sanguineus. Foram detectados DNA de Cercopithifilaria bainae (43,83%; 32/73), Ehrlichia canis (24,65%; 18/73), Anaplasma platys (19,17%; 14/73) e Hepatozoon canis (5,47%; 4/73). A identidade dos patógenos foi confirmada por análise de sequência de DNA. O presente estudo confirma a circulação dos haplótipos A e B de R. sanguineus no estado de Mato Grosso do Sul e sua importância como vetor de vários patógenos de interesse veterinário. Finalmente, este é o primeiro relato de C. bainae em carrapatos na região centro-oeste do Brasil.


Assuntos
Animais , Vetores Aracnídeos/parasitologia , Rhipicephalus sanguineus/parasitologia , Cães/parasitologia , Rickettsia/isolamento & purificação , Rickettsia/genética , Babesia/isolamento & purificação , Babesia/genética , Brasil , RNA Ribossômico 16S/genética , Eucoccidiida/isolamento & purificação , Eucoccidiida/genética , Ehrlichia canis/isolamento & purificação , Ehrlichia canis/genética , Anaplasma/isolamento & purificação , Anaplasma/genética
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